Journal: BMC Oral Health

Article Title: Expression profile of GSDMB in oral squamous cell carcinoma and its impact on tumor immune microenvironment and prognosis

doi: 10.1186/s12903-026-07781-1

Figure Lengend Snippet: Expression landscape of GSDMB across different groups (330 OSCC tissues, 32 normal tissues). A - C Differential expression of GSDMB among Grade categories, age groups, and N stages. D Boxplot showing the expression difference of GSDMB between OSCC cases and controls. E Receiver Operating Characteristic (ROC) curve for GSDMB. F Forest plot from univariate Cox regression analysis based on GSDMB expression levels and prognostic outcomes across various cancers. G Boxplot illustrating differences in GSDMB expression between control and disease groups across pan-cancer samples. “wilcox.test” is used to calculate the significance p-value (* p<0.05, ** p<0.01, *** p<0.001)

Article Snippet: The human OSCC cell lines SCC4 and SCC25 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Quantitative Proteomics, Control

Journal: BMC Oral Health

Article Title: Expression profile of GSDMB in oral squamous cell carcinoma and its impact on tumor immune microenvironment and prognosis

doi: 10.1186/s12903-026-07781-1

Figure Lengend Snippet: Landscape of Tumor Mutational Burden (TMB) in high and low GSDMB expression groups (318 OSCC tissues). A - B Waterfall plots depicting mutations in high and low GSDMB expression groups. C Boxplot comparing TMB differences between the two groups. D Scatter plot of the correlation between TMB and GSDMB expression levels. E Kaplan-Meier survival curves for high and low TMB groups. F KM survival curves for four sample groups classified by combined TMB and GSDMB expression levels

Article Snippet: The human OSCC cell lines SCC4 and SCC25 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing

Journal: BMC Oral Health

Article Title: Expression profile of GSDMB in oral squamous cell carcinoma and its impact on tumor immune microenvironment and prognosis

doi: 10.1186/s12903-026-07781-1

Figure Lengend Snippet: Analysis of differences between the two groups and GSEA analysis results (330 OSCC tissues). A Volcano plot displaying DEGs. B Heatmap of DEGs illustrating expression levels across samples. C Circle plot combined with a network view of GO/KEGG pathway enrichment analysis. D Bar plot summarizing the results of GO/KEGG enrichment analysis. E - L Gene set enrichment analysis (GSEA) results for key KEGG pathways. Specific pathways include ECM-receptor interaction ( E ), focal adhesion ( F ), MAPK signaling ( G ), natural killer cell-mediated cytotoxicity ( H ), primary immunodeficiency ( I ), T-cell receptor signaling ( J ), TGF-beta signaling ( K ), and Toll-like receptor signaling ( L ). Key statistics (NES: Normalized Enrichment Score; p-value; FDR: False Discovery Rate) are provided in the plots

Article Snippet: The human OSCC cell lines SCC4 and SCC25 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing

Journal: BMC Oral Health

Article Title: Expression profile of GSDMB in oral squamous cell carcinoma and its impact on tumor immune microenvironment and prognosis

doi: 10.1186/s12903-026-07781-1

Figure Lengend Snippet: Basic analysis results of scRNA-seq data. A Violin plots of six OSCC samples' scRNA-seq data post-merging and quality control, indicating the number of nonzero-expressed genes per cell, total UMI counts, and mitochondrial gene percentage. B Two-dimensional distribution of six samples with batch effects. C Distribution of different samples after removing batch effects using the "Harmony" package in R. D Heatmap predicting cell cluster identities using the "singleR" software package. E Two-dimensional distribution of different cell types. F Expression of GSDMB in different cell types. G Distribution of six OSCC samples across different cell types

Article Snippet: The human OSCC cell lines SCC4 and SCC25 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Software, Expressing

Journal: BMC Oral Health

Article Title: Expression profile of GSDMB in oral squamous cell carcinoma and its impact on tumor immune microenvironment and prognosis

doi: 10.1186/s12903-026-07781-1

Figure Lengend Snippet: Classification of high and low expression cell clusters and GSDMB expression in stRNA-seq data. A All cells were divided into high and low expression clusters based on the median value of GSDMB. B Proportions of different cell types within high and low expression clusters. C Results of GSEA analysis for the two clusters. D Clustering results for four slices based on OSCC stRNA-seq data. E Cell type annotation results for four slices from OSCC stRNA-seq data. F Expression of GSDMB in different cell types

Article Snippet: The human OSCC cell lines SCC4 and SCC25 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing

Journal: BMC Oral Health

Article Title: Expression profile of GSDMB in oral squamous cell carcinoma and its impact on tumor immune microenvironment and prognosis

doi: 10.1186/s12903-026-07781-1

Figure Lengend Snippet: GSDMB silencing attenuates the malignant properties and modulates 5-FU induced pyroptosis related responses in SCC25 cells. A IHC analysis of GSDMB expression in OSCC tissues and normal oral mucosa (n = 40). B – C Western blot analysis of GSDMB protein levels in HOK and OSCC cell lines and in SCC25 cells following siRNA transfection. D Cell viability assessed by the CCK-8 assay. E Representative images and quantification of colony formation. F Wound healing assay evaluating cell migration. G Transwell invasion assay. H Dose–response curves and corresponding IC50 values for 5-fluorouracil (5-FU). I Lactate dehydrogenase (LDH) release assay assessing 5-FU induced lytic cell death. J – K ELISA quantification of IL-1β and IL-18 levels in culture supernatants after 5-FU treatment. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: The human OSCC cell lines SCC4 and SCC25 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Western Blot, Transfection, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Dental Sciences

Article Title: APOBEC3B/ASF1B–TGF-β signaling axis promotes epithelial–mesenchymal transition in HPV-positive oropharyngeal cancer

doi: 10.1016/j.jds.2025.10.039

Figure Lengend Snippet: Functional characterization of APOBEC3B silencing and its downstream effects in vitro. (A) DepMap analysis identifying HPV-negative (SCC-4) and HPV-positive (SCC-154) oral squamous cell carcinoma lines with differential APOBEC3B expression suitable for functional validation. (B) Quantitative PCR analysis showing that APOBEC3B silencing significantly downregulates its downstream targets ASF1B and RPA2, indicating disruption of the APOBEC3B/ASF1B regulatory axis. (C–D) Cisplatin sensitivity, migration, and invasion assays demonstrate that APOBEC3B knockdown reduces cell motility and enhances cisplatin-induced cytotoxicity, as assessed by CellTiter-Glo viability assay (Promega) and Matrigel-based invasion analysis.(E) Tumor spheroid formation assay showing that shAPOBEC3B markedly suppresses both the number and size of tumor spheres in HPV-positive SCC-154 cells, indicating impaired self-renewal capacity and partial re-sensitization of irradiation-resistant cells to treatment.Data represent mean ± SD from three independent experiments; statistical significance was determined by Student's t-test ( P < 0.05). Abbreviations: SD, standard deviation; EMT, epithelial–mesenchymal transition; HPV, human papillomavirus; shRNA, short hairpin RNA.

Article Snippet: Human oropharyngeal squamous cell carcinoma cell lines SCC-4 (HPV-negative) and SCC-154 (HPV-positive) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Functional Assay, In Vitro, Expressing, Biomarker Discovery, Real-time Polymerase Chain Reaction, Disruption, Migration, Knockdown, Viability Assay, Tube Formation Assay, Irradiation, Standard Deviation, shRNA